RBS

Part:BBa_K2550200:Design

Designed by: Gaurav Byagathvalli   Group: iGEM18_Lambert_GA   (2018-10-15)


RBS for Toehold Switches


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the toehold switch, it is important to consider the preceding and succeeding sequences to the RBS to determine the optimal conformation for functionality.


Source

This part is derived from the Zika Toehold Switch biosensors developed by Pardee et al. The source can be viewed at: https://doi.org/10.1016/j.cell.2016.04.059.

References