RBS
Part:BBa_K2550200:Design
Designed by: Gaurav Byagathvalli Group: iGEM18_Lambert_GA (2018-10-15)
RBS for Toehold Switches
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When designing the toehold switch, it is important to consider the preceding and succeeding sequences to the RBS to determine the optimal conformation for functionality.
Source
This part is derived from the Zika Toehold Switch biosensors developed by Pardee et al. The source can be viewed at: https://doi.org/10.1016/j.cell.2016.04.059.